LipoKnoxa™ Human MFN2 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX501-1	GCAGGTTTACTGCGAGGAAAT	CDS	Inquiry
V0126XX501-2	GCTCAGTGCTTCATCCCATTT	3' UTR	Inquiry
V0126XX501-3	GCACTTTGTCACTGCCAAGAA	CDS	Inquiry
V0126XX501-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human MFN2 shRNA Lentiviral Particle (Silencing) targets mitofusin 2, which mediates mitochondrial fusion and insulin signaling. By offering both high-potency U6 systems and physiologically refined miR30-based shRNA, this tool helps researchers investigate mitochondrial dysfunction in obesity. Every preparation is subjected to total QC verification—covering functional titer, sterility, and mycoplasma testing—to ensure experimental reproducibility.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: MFN2

Full Name: Mitofusin 2

Gene Symbol: HSG; MSL; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B

Gene ID: 9927

RefSeq ID-1: NP_001121132.1

RefSeq ID-2: NM_001127660.2

Summary: MFN2 encodes a protein located in the mitochondrial membrane that facilitates mitochondrial fusion and supports the maintenance of the mitochondrial network. It also plays a role in regulating vascular smooth muscle cell proliferation and may influence obesity-related mechanisms. Mutations in this gene are linked to Charcot-Marie-Tooth disease type 2A2, hereditary motor and sensory neuropathy VI, and early-onset stroke. Two transcript variants producing the same protein have been identified.

LipoKnoxa™ Human MFN2 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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