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LipoKnoxa™ Human MAGEL2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX58
Species:
Human
Target Gene:
MAGEL2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX58-1 | CGCCTATATTATCATCAACAA | CDS | Inquiry |
| V0126XX58-2 | GCCCAATATGAATGCCTCAAA | CDS | Inquiry |
| V0126XX58-3 | CGAAGTGTTTGTCAGGCAGAA | CDS | Inquiry |
| V0126XX58-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human MAGEL2 shRNA Ad5 Particle (Silencing) targets the MAGEL2 gene, which is located in the Prader-Willi syndrome (PWS) chromosomal region and is vital for hypothalamic function and circadian rhythm. Utilizing an advanced RNAi platform with flexible shRNA architectures, this tool aids in uncovering the pathways behind syndromic obesity and sleep-disordered breathing. We provide a fully validated product through rigorous QC, ensuring sequence integrity and microbial safety.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
MAGEL2
Full Name:
MAGE family member L2
Gene Symbol:
PWLS; nM15; NDNL1; SHFYNG
Gene ID:
54551
RefSeq ID-1:
NP_061939.3
RefSeq ID-2:
NM_019066.5
Summary:
MAGEL2 is a paternally expressed, imprinted gene located within the Prader–Willi syndrome (PWS) critical region on chromosome 15q11–q13. Loss of expression of genes in this region leads to PWS, a disorder characterized by neonatal hypotonia, developmental delay, hyperphagia, and childhood-onset obesity. MAGEL2 shares structural similarity with the neuronal differentiation factor necdin (NDN) and is thought to contribute to hypothalamic dysfunction underlying the obesity phenotype observed in PWS.
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