LipoKnoxa™ Human IL10 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX538-1	GCTGGACAACTTGTTGTTAAA	CDS	Inquiry
V0126XX538-2	GGGTGACACACTATGGTATTT	3' UTR	Inquiry
V0126XX538-3	GCAGGTGAAGAATGCCTTTAA	CDS	Inquiry
V0126XX538-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human IL10 shRNA Lentiviral Particle (Silencing) facilitates the specific inhibition of interleukin-10 to study its anti-inflammatory role in fat tissue. Developed using optimized U6 and miR30-based shRNA systems, this tool allows for stable integration and reliable knockdown. Every batch is validated through total quality control screening for functional titer and microbial purity.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: IL10

Full Name: Interleukin 10

Gene Symbol: CSIF; TGIF; GVHDS; IL-10; IL10A

Gene ID: 3586

RefSeq ID-1: NP_000563.1

RefSeq ID-2: NM_000572.3

Summary: IL10 encodes a potent anti-inflammatory cytokine that modulates immune responses by suppressing pro-inflammatory signaling pathways. By limiting chronic inflammation, IL-10 indirectly supports insulin sensitivity and metabolic homeostasis. Dysregulation of IL-10 signaling has been linked to obesity-associated inflammatory states.

LipoKnoxa™ Human IL10 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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