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LipoKnoxa™ Human BBS4 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX48
Species:
Human
Target Gene:
BBS4
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX48-1 | GCACGATCTGACTTATATAAT | CDS | Inquiry |
| V0126XX48-2 | ACCTAGGAGTTTGCTACATAT | CDS | Inquiry |
| V0126XX48-3 | TGACCAATCTGGAAGATATAG | CDS | Inquiry |
| V0126XX48-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human BBS4 shRNA Ad5 Particle (Silencing) focuses on BBS4, which bridges the BBSome and microtubule motor machinery. Knocking down this gene is critical for investigating the transport of metabolic receptors to the primary cilium. Our customizable platform supports flexible reporter gene inclusion and ensures high viral potency through rigorous QC screening for sterility and mycoplasma under a U6 or miR30 shRNA framework.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
BBS4
Full Name:
Bardet-Biedl syndrome 4
Gene ID:
585
RefSeq ID-1:
NP_001239607.1
RefSeq ID-2:
NM_001252678.2
Summary:
BBS4 is a member of the Bardet–Biedl syndrome gene family and is implicated in an autosomal recessive disorder featuring obesity, pigmentary retinopathy, polydactyly, renal anomalies, and cognitive deficits. BBS4 participates in cilia formation and function and localizes to basal bodies, ciliary axonemes, and pericentriolar regions. The protein shares sequence similarity with O-linked N-acetylglucosamine transferases and forms part of the multi-protein BBSome complex. Alternative splicing gives rise to multiple transcript variants.
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