LipoKnoxa™ Human BBIP1 shRNA Lentiviral Particle (Silencing)

Product Overview

Description: LipoKnoxa™ Human BBIP1 shRNA Lentiviral Particle (Silencing) targets BBIP1, required for ciliary function and linked to obesity syndromes. This lentiviral tool is developed using both U6 and miR30-based shRNA systems, offering stable knockdown in both dividing and non-dividing cells. Every batch is subjected to total QC verification, including sequence accuracy and microbial safety checks, ensuring reproducible results for long-term ciliary research.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: BBIP1

Full Name: BBSome interacting protein 1

Gene Symbol: BBS18; BBIP10; bA348N5.3; NCRNA00081

Gene ID: 92482

RefSeq ID-1: NP_001182233.1

RefSeq ID-2: NM_001195304.2

Summary: BBIP1 (formerly BBS18) encodes a protein that is an essential component and one of the eight subunits required for the complete assembly of the BBSome complex. This complex is vital for the correct trafficking of signaling receptors (such as the somatostatin receptor) to and from the primary cilium, which is the cellular antenna coordinating key developmental and metabolic pathways. Mutations in BBIP1 result in Bardet-Biedl syndrome type 18, underscoring the necessity of this subunit for ciliary function and the prevention of the BBS phenotype, which universally includes severe obesity.

LipoKnoxa™ Human BBIP1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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