LipoKnoxa™ Human AKT1 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX519-1	CGTGCCATGATCTGTATTTAA	3' UTR	Inquiry
V0126XX519-2	GGACAAGGACGGGCACATTAA	CDS	Inquiry
V0126XX519-3	CGCGTGACCATGAACGAGTTT	CDS	Inquiry
V0126XX519-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human AKT1 shRNA Lentiviral Particle (Silencing) targets the AKT1 kinase to investigate its role in cellular growth and survival in metabolic tissues. Engineered with both U6 and miR30-based shRNA frameworks, this tool allows for stable gene silencing in difficult-to-transduce cells. Every batch is subjected to rigorous quality control, including functional titer, sterility, and mycoplasma testing.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: AKT1

Full Name: AKT serine/threonine kinase 1

Gene Symbol: AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA

Gene ID: 207

RefSeq ID-1: NP_001014431.1

RefSeq ID-2: NM_001014431.2

Summary: AKT1 is a central signaling node within the PI3K/AKT/mTOR pathway, which integrates signals from insulin and growth factors. It regulates a vast array of functions, including glucose metabolism, cell proliferation, and survival. While its overactivation is frequently linked to cancer and overgrowth syndromes, its canonical metabolic role is to promote glucose uptake and protein synthesis, making it essential for systemic nutrient management.

LipoKnoxa™ Human AKT1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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