LipoKnoxa™ Human AKT2 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX500-1	CAAATGGACACGGCATCATAA	3' UTR	Inquiry
V0126XX500-2	AGTCTACCCTGGTGGTCATAA	3' UTR	Inquiry
V0126XX500-3	TGCGGAAGGAAGTCATCATTG	CDS	Inquiry
V0126XX500-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human AKT2 shRNA Lentiviral Particle (Silencing) facilitates the persistent knockdown of the AKT2 isoform, which is essential for insulin-regulated glucose transport. Developed through an advanced RNAi platform with a choice of U6 or miR30-based systems, this tool enables in-depth metabolic pathway analysis. Rigorous QC protocols, including functional titering and microbial testing, are conducted on every batch to ensure experimental safety.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: AKT2

Full Name: AKT serine/threonine kinase 2

Gene Symbol: PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Gene ID: 208

RefSeq ID-1: NP_001317440.1

RefSeq ID-2: NM_001330511.1

Summary: AKT2 encodes a serine/threonine kinase involved in insulin signaling and other cellular pathways. It functions as a general protein kinase and has been implicated in glucose uptake and energy homeostasis. Overexpression contributes to cancer progression, while normal AKT2 activity is crucial for regulating metabolism and obesity susceptibility.

LipoKnoxa™ Human AKT2 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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