Human obestatin ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1202

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: Obestatin

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Human obestatin ELISA kit-Quantitative sandwich

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Product Overview

Description: Human obestatin ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of obestatin in human samples with a range of 78-5000 pg/mL and a minimum detectable dose (sensitivity) of 32 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The methodology applied in this assay is the sandwich enzyme immunoassay format. A microtiter plate has been pre-coated with an antibody specific to OB. Standards or samples are added to the appropriate plate wells together with a biotin-labeled antibody specific for OB. Next, avidin conjugated to HRP is introduced into each microplate well and incubated. Upon the addition of TMB substrate solution, a color change will be observed only in wells containing the complete sandwich complex (OB, biotinylated antibody, and enzyme-labeled avidin). The reaction is halted by adding a sulphuric acid solution, and the color shift is measured at a wavelength of 450 nm ± 10 nm. The final concentration of OB in the specimens is calculated by mapping the sample O.D. values to the standard curve.

Background: Obestatin is a hormone secreted by cells in the stomach and small intestine that may regulate both hunger and satiety signals through a complex balancing system. Studying how obestatin interacts with other hormones, such as insulin and leptin, can help us gain a more comprehensive understanding of energy metabolism and analyze its role in obesity.

Synonyms: OB

Applications: Human obestatin ELISA kit-Quantitative sandwich is used to quantify obestatin in serum, plasma, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolic, endocrinology, obesity, etc.

Research Area: Metabolic; Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 78-5000 pg/mL

Sensitivity: 32 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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