Mouse fatty acid (FA) ELISA kit

Cat. No.: OB0625WXX-787

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: FA

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Product Overview

Description: Mouse fatty acid (FA) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FA in mouse with a range of 3.12-200 nmol/mL and a specificity of up to 0.6 nmol/mL.

Assay Principle: The pre-coated antibody is a mouse FA monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. The sample and biotin-labeled antibody are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells. The reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.

Background: Fatty acids (FAs) are carboxylic acids with an aliphatic chain and are diverse. Their metabolism can serve as a target for obesity treatment.

Synonyms: Carboxylic acid

Applications: Mouse fatty acid (FA) ELISA kit is used to quantify FA in mouse serum, plasma, cell culture supernatant samples of mouse, providing data to support a wide range of studies.

Research Area: Lipid metabolism; Obesity research

Specification

Sample Type: Mouse serum; Plasma; Cell culture supernatant

Detection Range: 3.12-200 nmol/mL

Sensitivity: Up to 0.6 nmol/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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