Bovine glucagon-like peptide 2 (GLP-2) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-202

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: GLP-2

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Bovine glucagon-like peptide 2 (GLP-2) ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine glucagon-like peptide 2 (GLP-2) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GLP-2 in bovine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of up to 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs a double-sandwich ELISA technique. The microplate is pre-coated with a bovine GLP-2 monoclonal antibody. Samples and a biotin-labeled polyclonal detection antibody are added to the wells and subsequently washed with PBS or TBS. Avidin-peroxidase conjugates are then added. Following a thorough wash, TMB substrate is introduced; the HRP-catalyzed reaction produces a blue product that turns yellow upon the addition of acid. The color intensity is positively correlated with the concentration of GLP-2 in the samples.

Background: GLP-2, as an incretin, primarily functions to promote intestinal growth and nutrient absorption. Recent studies have revealed that GLP-2's association with obesity primarily manifests through its regulatory effects on intestinal barrier function and the gut microbiome. By improving gut health, GLP-2 may indirectly influence metabolism. Furthermore, ongoing research is exploring the potential applications of GLP-2 and its analogues in regulating fat metabolism and energy expenditure, offering new avenues for obesity treatment.

Synonyms: Glp2; GLP2

Applications: Bovine glucagon-like peptide 2 (GLP-2) ELISA kit (0.156-10 ng/mL) is used to quantify GLP-2 in bovine serum, plasma, and cell culture supernatant of bovine, providing data to support research in a wide range of areas, including metabolism, endocrinology, cardiovascular biology, obesity, and others.

Research Area: Metabolism; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Bovine serum; Plasma; Cell culture supernatant

Detection Range: 0.156-10 ng/mL

Sensitivity: Up to 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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