Dog peroxisome proliferator-activated receptor α (PPARα) ELISA kit

Cat. No.: 0126WXX-1458

Assay Type: Quantitative ELISA

Target Species: Dog

Assay Target: PPARα

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Dog peroxisome proliferator-activated receptor α (PPARα) ELISA kit

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Product Overview

Description: Dog peroxisome proliferator-activated receptor α (PPARα) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in dog samples with a range of 78.0-5000 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to PPARα. Standards or samples are added to the appropriate wells along with a biotin-conjugated polyclonal detection antibody and an avidin-HRP conjugate. Following incubation and a wash step, a TMB substrate solution is added to each well. Only wells containing the PPARα complex will exhibit a color change. The enzyme-substrate reaction is terminated by a sulphuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of PPARα is determined by comparing the O.D. of the samples to the standard curve.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Dog peroxisome proliferator-activated receptor α (PPARα) ELISA kit is used to quantify PPARα in serum, plasma, tissue homogenates, cell culture supernatant, and other biological fluids of dog, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernatants; Other biological fluids

Detection Range: 78.0-5000 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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