Goat leptin (LEP) ELISA kit (0.625-40 ng/mL)

Cat. No.: 0126WXX-800

Assay Type: Quantitative sandwich ELISA

Target Species: Goat

Assay Target: LEP

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Goat leptin (LEP) ELISA kit (0.625-40 ng/mL)

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Product Overview

Description: Goat leptin (LEP) ELISA kit (0.625-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in goat samples with a range of 0.625-40 ng/mL and a minimum detectable dose (sensitivity) of <0.156 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. A microplate is pre-coated with an antibody specific for LEP. Standards and samples are added to the wells where the immobilized antibody binds any LEP present. After washing, a biotin-conjugated detection antibody and avidin-HRP are added. Following a final wash cycle, a substrate solution is introduced, causing color development in direct proportion to the amount of bound LEP. The reaction is stopped, and the color intensity is measured at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Goat leptin (LEP) ELISA kit (0.625-40 ng/mL) is used to quantify LEP in serum and plasma of goat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma

Detection Range: 0.625-40 ng/mL

Sensitivity: <0.156 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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