Rat NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1808

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: SIRT1

Size:

Rat NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Rat NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of SIRT1 in rat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.094 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA method. The micro ELISA plate is pre-coated with an antibody specific to SIRT1. Standards or samples are added to the wells to combine with the immobilized antibody. Then, a biotinylated detection antibody specific for SIRT1 and an avidin-HRP conjugate are added sequentially and incubated. After washing away free components, a substrate solution is introduced. Only wells containing the SIRT1, biotinylated antibody, and avidin-HRP conjugate will appear blue. The reaction is terminated by a sulphuric acid solution, turning the product yellow. The OD is measured at 450 nm ± 2 nm, and the SIRT1 concentration is determined by comparing the sample OD to the standard curve.

Background: Sirtuin 1 (SIRT1) is an NAD+-dependent protein deacetylase that links cellular energy status to transcriptional regulation, modulating processes such as metabolism, autophagy, apoptosis, and cell cycle control. SIRT1 deacetylates a variety of transcription factors and coregulators, influencing gene expression in liver, muscle, and adipose tissue, and participates in chromatin silencing through energy-dependent complexes. By sensing NAD+/NADH ratios, SIRT1 integrates metabolic signals and contributes to the regulation of glucose and lipid homeostasis, as well as cellular responses to stress and nutrient availability.

Synonyms: SIR2L1; Silent mating type information regulation 2 homolog 1; SIR2-like protein 1; SirtT1 75 kDa fragment; Sirtuin 1

Applications: Rat NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit-Quantitative sandwich is used to quantify SIRT1 in serum, plasma, and biological fluids of rats, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.094 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out