Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL)

Cat. No.: OB0625WXX-640

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ENPP1

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Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL)

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Product Overview

Description: Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ENPP1 in human with a range of 0.156-10 ng/mL and a specificity of 0.061 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an antibody specific for ENPP1. Standards or samples are then added to the corresponding microtiter plate wells along with the biotin-conjugated specific ENPP1 antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. Upon addition of the TMB substrate solution, only wells containing ENPP1, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ENPP1 in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: ENPP1 is a member of the ENPP family, which has broad specificity and cleaves various substrates. It is primarily involved in ATP hydrolysis at the plasma membrane, playing a role in regulating pyrophosphate levels, bone mineralization, and soft tissue calcification.

Synonyms: Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1

Applications: Human ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP1) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL) is used to quantify ENPP1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Bone mineralization and soft tissue calcification research; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Method: Enzyme and Kinase

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.061 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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