Rat visceral adipose-specific serine protease inhibitor; vaspin ELISA kit

Cat. No.: 0126WXX-1779

Assay Type: Quantitative ELISA

Target Species: Rat

Assay Target: Vaspin

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Rat visceral adipose-specific serine protease inhibitor; vaspin ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat visceral adipose-specific serine protease inhibitor; vaspin ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of vaspin in rat samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of 7.8 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vaspin. Standards or samples are added to the designated microtiter wells with a biotin-conjugated antibody preparation specific for vaspin. Subsequently, Avidin-conjugated HRP is added and incubated. A TMB substrate solution is then added to each well. Only wells containing the vaspin-biotin-avidin complex will demonstrate a colorimetric change. A sulphuric acid solution terminates the reaction, and the absorbance is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of vaspin is determined by comparing the OD of the samples to the standard curve.

Background: Vaspin is an adipokine secreted by visceral adipose tissue. It was initially identified as a serine protease inhibitor. In obesity research, Vaspin is recognized as a protective factor with insulin-sensitizing effects. Vaspin levels typically correlate with the degree of insulin resistance and are considered a molecular link between visceral fat function and systemic metabolic health.

Synonyms: SERPINA12

Applications: Rat visceral adipose-specific serine protease inhibitor, vaspin ELISA kit is used to quantify vaspin in serum, plasma, and tissue homogenates of rats, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 31.25-2000 pg/mL

Sensitivity: 7.8 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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