Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) ELISA kit (0.625-20 ng/mL)

Cat. No.: 0126WXX-1059

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: NAPEPLD

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Human <i>N</i>-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) ELISA kit (0.625-20 ng/mL)

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Product Overview

Description: Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) ELISA kit (0.625-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NAPEPLD in human samples with a range of 0.625-20 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on NAPEPLD antibody-NAPEPLD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAPEPLD antigen targets in samples.

Background: NAPEPLD is a phospholipase D and a key rate-limiting enzyme in the endogenous cannabinoid synthesis pathway. The endocannabinoid system (ECS) is a central and peripheral regulatory system governing appetite, lipid synthesis, and energy balance. Endocannabinoids produced by NAPEPLD activate CB1 receptors, typically leading to increased appetite and enhanced lipogenesis. Thus, NAPEPLD is a key enzyme in lipid metabolism pathways influencing the obesity process.

Synonyms: NAPE-PLD; N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D

EC NO.: 3.1.4.54

Applications: Human N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD) ELISA kit (0.625-20 ng/mL) is used to quantify NAPEPLD in undiluted original human serum, plasma, and tissue homogenates of human, providing data to support research in a wide range of areas, including appetite regulation, obesity, etc.

Research Area: Appetite regulation; Obesity

Specification

Sample Type: Undiluted original human serum; Plasma; Tissue homogenates

Detection Range: 0.625-20 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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