Rat lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-883

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LPL

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Rat lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in rat samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.114 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on LPL antibody-LPL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LPL antigen targets in samples.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Rat lipoprotein lipase (LPL) ELISA kit (0.312-20 ng/mL) is used to quantify LPL in serum, plasma, tissue homogenates, and cell lysates, cell culture supernatants, and other biological fluids of rat, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.114 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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