Human glucagon (GCG) ELISA kit (7.813-500 pg/mL)

Cat. No.: 0126WXX-65

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: GCG

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Human glucagon (GCG) ELISA kit (7.813-500 pg/mL)

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Product Overview

Description: Human glucagon (GCG) ELISA kit (7.813-500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in human samples with a range of 7.813-500 pg/mL and a minimum detectable dose (sensitivity) of 4.688 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes sandwich ELISA technology. A capture antibody is pre-coated onto 96-well plates. Standards, samples, and a biotin-conjugated detection antibody are added sequentially. After washing with a wash buffer, HRP-streptavidin is added, and unbound conjugates are washed away. TMB substrate is used to visualize the HRP enzymatic reaction, producing a blue product that turns yellow after adding the acidic stop solution. The intensity of the yellow color is directly proportional to the amount of target captured. Absorbance is read at 450 nm, and the target concentration is calculated accordingly.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Human glucagon (GCG) ELISA kit (7.813-500 pg/mL) is used to quantify GCG in serum, plasma, cell culture supernatant, cell, tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell; Tissue lysate; Other liquid samples

Detection Range: 7.813-500 pg/mL

Sensitivity: 4.688 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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