Rat glucagon (GCG) ELISA kit (18.52-1500 pg/mL)

Cat. No.: 0126WXX-78

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: GCG

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Rat glucagon (GCG) ELISA kit (18.52-1500 pg/mL)

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Product Overview

Description: Rat glucagon (GCG) ELISA kit (18.52-1500 pg/mL, 8.15 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in rat samples with a range of 18.52-1500 pg/mL and a minimum detectable dose (sensitivity) of 8.15 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GCG has been pre-immobilized onto a microplate. A competitive inhibition reaction is initiated between biotin-labeled GCG and unlabeled GCG (standards or samples) for the binding sites on the pre-coated antibody. After incubation, the unbound components are washed away. Subsequently, Avidin-conjugated HRP is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of GCG in the sample. Upon the addition of the substrate solution, the intensity of the color developed remains inversely proportional to the GCG concentration. The final absorbance is measured spectrophotometrically to determine the concentration of GCG in the specimens.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Rat glucagon (GCG) ELISA kit (18.52-1500 pg/mL, 8.15 pg/mL) is used to quantify GCG in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of rat, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 18.52-1500 pg/mL

Sensitivity: 8.15 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 97-95%

Storage: Store at 2-8°C.

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