Human apolipoprotein C-II (APOC2) ELISA kit (3.125-200 ng/mL)

Cat. No.: OB0625WXX-123

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: APOC2

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Human apolipoprotein C-II (APOC2) ELISA kit (3.125-200 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human apolipoprotein C-II (APOC2) ELISA kit (3.125-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of APOC2 in human with a range of 3.125-200 ng/mL and a specificity of 1.875 ng/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific for human APOC2. Standards or samples are added to the appropriate micro ELISA plate wells and bound to the specific antibody. A biotinylated detection antibody specific for human APOC2 and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing human APOC2, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color turns yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of human APOC2. The concentration of APOC2 in the sample can be calculated by comparing the OD value of the sample with the standard curve.

Background: Apolipoprotein C-II is a component of very low-density lipoproteins and chylomicrons. It activates lipoprotein lipase and plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.

Synonyms: Apolipoprotein C2; APC2; APO-CII; APOC-II; Apo-C2; ProapoC-II; Proapolipoprotein C-II

Applications: Human apolipoprotein C-II (APOC2) ELISA kit (3.125-200 ng/mL) is used to quantify APOC2 in serum, plasma, and biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Metabolic pathway; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 3.125-200 ng/mL

Sensitivity: 1.875 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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