Human pro-opiomelanocortin (POMC) ELISA kit (123.5-10000 pg/mL)

Cat. No.: 0126WXX-1431

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: POMC

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Human pro-opiomelanocortin (POMC) ELISA kit (123.5-10000 pg/mL)

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Product Overview

Description: Human pro-opiomelanocortin (POMC) ELISA kit (123.5-10000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in human samples with a range of 123.5-10000 pg/mL and a minimum detectable dose (sensitivity) of 51.7 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to POMC is pre-coated onto the microplate. A competitive reaction is initiated between biotin-labeled POMC and unlabeled POMC (standards or samples) for the limited binding sites on the immobilized antibody. After incubation, unbound conjugates are washed away, and avidin-conjugated HRP is added. Upon the addition of the substrate solution, the resulting color intensity is inversely proportional to the concentration of POMC in the sample.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Human pro-opiomelanocortin (POMC) ELISA kit (123.5-10000 pg/mL) is used to quantify POMC in serum, plasma, and other biological fluids of human, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 123.5-10000 pg/mL

Sensitivity: 51.7 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 83-104%

Storage: Store at 2-8°C.

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