Canine leptin (LEP) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-792

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: LEP

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Canine leptin (LEP) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Canine leptin (LEP) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in canine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a double-antibody sandwich ELISA technique. The microplate is pre-coated with a canine LEP monoclonal antibody, while a biotin-labeled polyclonal antibody serves as the detection agent. Samples and detection antibodies are incubated in the wells and washed with PBS or TBS. Avidin-peroxidase conjugates are then added. Following a final wash, a TMB substrate facilitates color development, shifting from blue to yellow under acidic conditions. The resulting color depth is positively correlated with the concentration of the target factors in the sample.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Canine leptin (LEP) ELISA kit (0.156-10 ng/mL)is used to quantify LEP in serum, plasma, and cell culture supernatant of canine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Canine serum; Plasma; Cell culture supernatant

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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