Products
- Anti-Obesity Compound Library
- GPCR/G Protein-Targeted Compounds
- Immunology/Inflammation-Targeted Compounds
- JAK/STAT-Targeted Compounds
- MAPK-Targeted Compounds
- Membrane Transporter/Ion Channel-Targeted Compounds
- Metabolism-Targeted Compounds
- NF-κB-Targeted Compounds
- Microbiology/Virology-Targeted Compounds
- Neuronal Signaling-Targeted Compounds
- PI3K/Akt/mTOR-Targeted Compounds
- Oxidation-reduction-Targeted Compounds
- Proteases/Proteasome-Targeted Compounds
- Stem Cells/Wnt-Targeted Compounds
- Tyrosine Kinase/Adaptors-Targeted Compounds
- Ubiquitin-Targeted Compounds
Online Inquiry
Rat constitutive androstane receptor (CAR) ELISA kit (0.156-10 ng/mL, 0.060 ng/mL)
Cat. No.:
OB0625WXX-243
Assay Type:
Quantitative sandwich ELISA
Target Species:
Rat
Assay Target:
CAR
Size:
Add to basket
Product Overview
Description:
Rat constitutive androstane receptor (CAR) ELISA kit (0.156-10 ng/mL, 0.060 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CAR in rat with a range of 0.156-10 ng/mL and a specificity of 0.060 ng/mL.
Assay Principle:
The microtiter plates in this kit are pre-coated with an antibody specific to CAR. Standards or samples are added to the corresponding microtiter plate wells and the biotin-conjugated CAR-specific antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. Upon addition of TMB substrate solution, only wells containing CAR, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of CAR in the sample is then determined by comparing the OD of the sample to a standard curve.
Background:
CAR is a nuclear receptor and transcription factor primarily known for regulating genes involved in drug metabolism and detoxification.
Synonyms:
NR1I3; NR1-I3; CAR1; MB67; Nuclear receptor subfamily 1, group I, Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67
Applications:
Rat constitutive androstane receptor (CAR) ELISA kit (0.156-10 ng/mL, 0.060 ng/mL) is used to quantify CAR in tissue homogenates, cell lysates, and other biological fluid samples of rat, providing data to support a wide range of studies.
Research Area:
Drug metabolism
Specification
Sample Type:
Tissue homogenates; Cell lysates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.060 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
