Human fatty acid synthase (FAS) ELISA kit (1.56-100 ng/mL)

Cat. No.: OB0625WXX-861

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: FAS

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Human fatty acid synthase (FAS) ELISA kit (1.56-100 ng/mL)

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Product Overview

Description: Human fatty acid synthase (FAS) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in human with a range of 1.56-100 ng/mL and a specificity of 0.39 ng/mL.

Assay Principle: An antibody specific for FAS has been pre-coated onto the microtiter plate. Standards and samples are pipetted into the wells, and the immobilized antibody binds to the FAS therein. After removing unbound material, a biotin-conjugated antibody specific for FAS is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of FAS bound in the first step. The color development is stopped, and the intensity of the color is measured.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Human fatty acid synthase (FAS) ELISA kit (1.56-100 ng/mL) is used to quantify FAS in serum, plasma, tissue homogenates, and cell lysate samples of human, providing data to support a wide range of studies.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates

Detection Range: 1.56-100 ng/mL

Sensitivity: 0.39 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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