Pig fatty acid synthase (FAS) ELISA kit

Cat. No.: OB0625WXX-844

Assay Type: Quantitative sandwich ELISA

Target Species: Pig

Assay Target: FAS

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Product Overview

Description: Pig fatty acid synthase (FAS) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAS in pig. The kit is highly sensitive and easy to use.

Assay Principle: An antibody specific for FAS has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any FAS is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for FAS is added to the wells. After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of FAS bound in the first step. The color development is stopped, and the intensity of the color is measured.

Background: FAS is a multi-enzyme protein that catalyzes fatty acid synthesis. It is essential for the de novo synthesis of long-chain saturated fatty acids from acetyl-CoA, malonyl-CoA, and NADPH. Studies have found that FAS may be involved in obesity by regulating feeding behavior and energy homeostasis, potentially playing a role in body weight regulation and obesity development.

Synonyms: Short chain dehydrogenase/reductase family 27X, Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase; OA519; SDR27X1; EC 2.3.1.85

EC NO.: 2.3.1.85

Applications: Pig fatty acid synthase (FAS) ELISA kit is an important tool for the quantitative detection of FAS in various biological samples (serum, plasma, and other biological fluids) of pigs.

Research Area: Fatty acid synthesis; Lipid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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