Pig 1-acylglycerol-3-phosphate O-acyltransferase (ABHD5) ELISA kit

Cat. No.: OB0525WXX-005

Assay Type: Quantitative sandwich ELISA

Target Species: Pig

Assay Target: ABHD5

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Pig 1-acylglycerol-3-phosphate <i>O</i>-acyltransferase (ABHD5) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Pig 1-acylglycerol-3-phosphate O-acyltransferase (ABHD5) ELISA kit is a ready-to-use ELISA kit for analyzing pig ABHD5 levels with high sensitivity and excellent specificity.

Assay Principle: An antibody specific for ABHD5 has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ABHD5 is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for ABHD5 is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of ABHD5 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Background: ABHD5 is a lysophosphatidic acid acyltransferase that plays a role in the biosynthesis of phosphatidic acid and lipid metabolism.

Synonyms: CGI-58; Abhydrolase domain-containing protein 5

Applications: Pig 1-acylglycerol-3-phosphate O-acyltransferase (ABHD5) ELISA kit is designed for the in vitro quantitative analysis of ABHD5 levels in pig serum, plasma, and other biological fluid samples.

Research Area: Lipid metabolism and biosynthesis; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Sample Volume: 1-200 μL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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