Human acetyl-CoA carboxylase (ACAC) ELISA kit (1.563-100 ng/mL, 0.94 ng/mL)

Cat. No.: OB0525WXX-087

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ACAC

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Human acetyl-CoA carboxylase (ACAC) ELISA kit (1.563-100 ng/mL, 0.94 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human acetyl-CoA carboxylase (ACAC) ELISA kit (1.563-100 ng/mL, 0.94 ng/mL) is a ready-to-use ELISA kit for analyzing human ACAC levels with a detection range of 1.563-100 ng/mL and a sensitivity of 0.94 ng/mL.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA method. The microtiter ELISA plates provided in the kit are pre-coated with antibodies specific to ACAC. Standards or samples are added to the corresponding wells of the ELISA plate and bound to the specific antibody. A biotinylated detection antibody specific for ACAC and avidin-HRP conjugate are then added sequentially to each microplate well and incubated. Free components are washed away, and substrate solution is added to each well. Only wells containing ACAC, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ACAC in the sample is calculated by comparing the OD value of the sample with the standard curve.

Background: ACAC (also known as ACC) is the enzyme that catalyzes the carboxylation of acetyl coenzyme A to produce malonyl coenzyme A, which is involved in fatty acid biosynthesis and β-oxidation. It is a potential target for the treatment of obesity and related diseases.

Synonyms: ACC; EC 6.4.1.2

Applications: Human acetyl-CoA carboxylase (ACAC) ELISA kit (1.563-100 ng/mL, 0.94 ng/mL) is designed for the in vitro quantitative analysis of ACAC levels in human serum, plasma, and biological fluid samples.

Research Area: Metabolites; Fatty acid biosynthesis; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 1.563-100 ng/mL

Sensitivity: 0.94 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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