AdipoUpX™ Human THBS1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human THBS1 MSCV Particle (Overexpression) enables stable overexpression of the human THBS1 gene. THBS1 encodes thrombospondin-1, an extracellular matrix protein involved in inflammation, angiogenesis, and insulin resistance, processes closely linked to obesity and metabolic dysfunction. This MSCV system supports sustained expression in stem cells and other mammalian cell lines used for metabolic studies. Product quality is ensured by comprehensive testing for viral titer, sterility, and mycoplasma contamination.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: THBS1

Full Name: Thrombospondin 1

Gene Symbol: TSP; THBS; TSP1; TSP-1; THBS-1

Gene ID: 7057

RefSeq ID-1: NP_003237.2

RefSeq ID-2: NM_003246.3

Summary: THBS1 (Thrombospondin-1) encodes a homotrimeric adhesive glycoprotein that mediates critical interactions between cells and the extracellular matrix. It binds to various structural proteins, such as collagen and fibronectin, to influence angiogenesis, platelet aggregation, and tissue remodeling. In the context of obesity, THBS1 is often studied for its role in adipose tissue inflammation and the vascular changes associated with fat mass expansion.

AdipoUpX™ Human THBS1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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