Product Overview
Description:
AdipoUpX™ Human STEAP4 MMLV Particle (Overexpression) is a ready-to-use retroviral vector particle professionally engineered for the stable overexpression of the human STEAP4 gene. This metalloreductase is a key regulator of adipocyte inflammation and iron homeostasis, making it essential for studying insulin resistance mechanisms. By integrating stably into the genome of dividing cells, this product facilitates the creation of permanent cell models for long-term functional studies. Every batch undergoes comprehensive quality control, including titer, sterility testing, and mycoplasma detection, to ensure the repeatability and safety of experimental results.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
STEAP4
Full Name:
STEAP4 metalloreductase
Gene Symbol:
TIARP; STAMP2; SchLAH; TNFAIP9
Summary:
STEAP4 encodes a Golgi-associated member of the six-transmembrane epithelial antigen of prostate (STEAP) family with NAD⁺-dependent metalloreductase activity. The protein facilitates intracellular iron and copper redox balance by converting ferric and cupric ions to their reduced forms. Experimental evidence from human and animal studies suggests a role for STEAP4 in adipocyte differentiation, lipid metabolism, and inflammatory regulation, supporting its involvement in obesity-related metabolic processes. Multiple alternatively spliced isoforms have been reported.
