AdipoUpX™ Human PRKACA MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PRKACA MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle professionally engineered for the stable overexpression of the human PRKACA gene. This catalytic subunit of PKA is a central node in lipolysis and hormonal signaling in adipocytes. Stable genomic integration into dividing cells allows for the longitudinal study of cAMP-mediated metabolic switches. Every batch passes comprehensive quality control, and our modular design supports flexible customization of reporter genes for precise tracking.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: PRKACA

Full Name: Protein kinase cAMP-activated catalytic subunit alpha

Gene Symbol: CAFD1; PKACA; PPNAD4

Gene ID: 5566

RefSeq ID-1: NP_001291278.1

RefSeq ID-2: NM_001304349.1

Summary: PRKACA encodes a catalytic subunit of cAMP-dependent protein kinase A, a key mediator of hormonal signaling. PKA activity regulates lipolysis, glucose metabolism, and endocrine responses, linking PRKACA to energy balance and obesity-related hormonal dysregulation.

AdipoUpX™ Human PRKACA MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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