AdipoUpX™ Human KCNJ5 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human KCNJ5 MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human KCNJ5 gene. This potassium channel is critical for researching the electrical excitability of endocrine cells and their link to aldosterone-related hypertension in obesity. The viral system offers high flexibility for the customization of functional markers. Every lot is verified through comprehensive quality control (titer, sterility, and mycoplasma detection) to ensure high-fidelity results in metabolic research.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: KCNJ5

Full Name: Potassium inwardly rectifying channel subfamily J member 5

Gene Symbol: CIR; GIRK4; KATP1; LQT13; KIR3.4

Gene ID: 3762

RefSeq ID-1: NP_000881.3

RefSeq ID-2: NM_000890.3

Summary: KCNJ5 encodes an inward-rectifier potassium channel subunit regulated by G-proteins, affecting cellular excitability. Mutations in this gene influence aldosterone secretion, which can impact electrolyte balance and energy homeostasis, thereby indirectly affecting body weight regulation.

AdipoUpX™ Human KCNJ5 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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