AdipoUpX™ Human APOE MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human APOE MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human APOE gene. This protein is essential for the normal catabolism of triglyceride-rich lipoprotein constituents and is a major focus in obesity and neuro-metabolic research. The MMLV vector ensures long-lasting overexpression in dividing host cells. Product quality is ensured by rigorous purification, titer validation, and comprehensive QC screening to provide a safe, repeatable, and reliable research tool.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: APOE

Full Name: Apolipoprotein E

Gene Symbol: AD2; LPG; APO-E; ApoE4; LDLCQ5

Gene ID: 348

RefSeq ID-1: NP_000032.1

RefSeq ID-2: NM_000041.4

Summary: APOE encodes a major apolipoprotein that is essential for the normal catabolism (breakdown and clearance) of triglyceride-rich lipoproteins, notably chylomicron and VLDL remnants. APOE binds to specific receptors on the liver and peripheral cells, acting as the recognition marker necessary for cellular uptake. This gene resides in a cluster on chromosome 19 with other related apolipoproteins (C1 and C2). Specific mutations in APOE result in familial dysbetalipoproteinemia (Type III hyperlipoproteinemia), a disorder characterized by the impaired clearance of these remnants, leading to elevated plasma cholesterol and triglyceride levels.

AdipoUpX™ Human APOE MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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