Product Overview
Description:
AdipoUpX™ Human MAP3K8 Ad5 Adenoviral Particle (Overexpression) is engineered for efficient transient overexpression of the human MAP3K8 gene in mammalian cells. MAP3K8 is a serine/threonine kinase involved in inflammatory signaling pathways, including ERK and NF-κB activation. This product enables functional studies of obesity-associated inflammation, adipose tissue remodeling, and metabolic dysfunction. Flexible reporter gene options and rigorous quality control, including titer, sterility, and mycoplasma testing, are provided.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
MAP3K8
Full Name:
Mitogen-activated protein kinase kinase kinase 8
Gene Symbol:
COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
Summary:
MAP3K8 encodes a serine/threonine kinase that functions as an upstream regulator of multiple intracellular signaling cascades, including the MAPK and JNK pathways. The protein is primarily localized in the cytoplasm and contributes to inflammatory and immune responses through activation of IκB kinases and subsequent NF-κB signaling. It has also been reported to enhance TNF-α and IL-2 production during T-cell activation. Alternative translation initiation and transcript processing can generate distinct isoforms, including a shorter form with reduced transforming activity.
