AdipoUpX™ Human KIF7 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human KIF7 Lentiviral Particle (Overexpression) is a powerful gene delivery tool specifically designed for the stable overexpression of the human KIF7 gene. This protein is vital for investigating ciliary Hedgehog signaling and its impact on developmental obesity syndromes. Genome integration facilitates the establishment of reliable cell lines for functional validation. All particles undergo rigorous quality control screening to ensure experimental reliability. Our customizable platform supports the flexible selection of fluorescent tags for real-time visualization of protein localization.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: KIF7

Full Name: Kinesin family member 7

Gene Symbol: ACLS; AGBK; HLS2; MMEDF; JBTS12; UNQ340

Gene ID: 374654

RefSeq ID-1: NP_940927.2

RefSeq ID-2: NM_198525.3

Summary: KIF7 encodes a cilia-associated kinesin protein that modulates the sonic hedgehog (SHH) pathway via GLI transcription factors. By influencing SHH signaling, KIF7 affects adipose tissue development and energy homeostasis. Mutations in KIF7 are associated with ciliopathies, some of which include obesity phenotypes.

AdipoUpX™ Human KIF7 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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