AdipoUpX™ Human IKBKB Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human IKBKB Lentiviral Particle (Overexpression) is a powerful gene delivery tool specifically designed for the stable overexpression of the human IKBKB (IKK-beta) gene. As a master switch for the NF-κB inflammatory pathway, it is an essential tool for establishing cell models that mimic the inflammatory state of obese tissues. Genome integration enables the creation of stable cell lines for high-throughput screening of anti-inflammatory compounds. All viral products undergo rigorous internal quality control to ensure reliability and efficacy.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: IKBKB

Full Name: Inhibitor of nuclear factor kappa B kinase subunit beta

Gene Symbol: IKK2; IKKB; IKK-2; IMD15; IMD15A; IMD15B; NFKBIKB; IKK-beta

Gene ID: 3551

RefSeq ID-1: NP_001177649.1

RefSeq ID-2: NM_001190720.2

Summary: IKBKB acts as the primary ignition switch for the NF-kappa-B inflammatory pathway. By phosphorylating the inhibitor of NF-kappa-B, it triggers the release and activation of this master transcription factor. This gene is a critical link between high-calorie diets and the "meta-inflammation" that leads to insulin resistance and chronic metabolic disease.

AdipoUpX™ Human IKBKB Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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