AdipoUpX™ Human IFT172 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human IFT172 MSCV Particle (Overexpression) provides a specialized platform for studying the role of intraflagellar transport in ciliary function and its impact on polygenic obesity. Overexpressing IFT172 allows researchers to explore how ciliary signaling modulates adipogenesis and energy balance. The MSCV system is highly efficient for gene delivery into stem cells, hematopoietic lineages, and various mammalian cell lines. To ensure the highest standards of safety and data consistency, these particles undergo exhaustive quality control encompassing titer measurement and microbial screening.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: IFT172

Full Name: Intraflagellar transport 172

Gene Symbol: SLB; wim; RP71; BBS20; osm-1; NPHP17; SRTD10

Gene ID: 26160

RefSeq ID-1: NP_056477.1

RefSeq ID-2: NM_015662.3

Summary: IFT172 produces a subunit of the intraflagellar transport subcomplex B (IFT-B), which is essential for the construction and maintenance of the primary cilium. Since the cilium acts as a sensory antenna for appetite and growth signals, mutations in IFT172 lead to ciliopathies often characterized by skeletal anomalies and syndromic obesity.

AdipoUpX™ Human IFT172 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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