AdipoUpX™ Human HMGCR MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HMGCR MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human HMGCR gene. As the rate-limiting enzyme in cholesterol biosynthesis, its stable expression is a primary focus for studying lipid homeostasis and statin sensitivity. The MMLV characteristics ensure the target gene integrates into the dividing cell genome for long-lasting study. Product quality is ensured by rigorous purification and comprehensive QC screening to provide a safe and repeatable research tool.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: HMGCR

Full Name: 3-Hydroxy-3-methylglutaryl-CoA reductase

Gene Symbol: MYPLG; LDLCQ3; LGMDR28

Gene ID: 3156

RefSeq ID-1: NP_000850.1

RefSeq ID-2: NM_000859.3

Summary: HMGCR encodes the rate-limiting enzyme in cholesterol biosynthesis, subject to negative feedback by sterols. As cholesterol metabolism is closely linked to lipid homeostasis and energy balance, HMGCR activity can influence obesity-related metabolic pathways. Multiple isoforms are produced via alternative splicing.

AdipoUpX™ Human HMGCR MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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