AdipoUpX™ Human CEP19 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human CEP19 Lentiviral Particle (Overexpression) is a professionally engineered vector particle providing efficient and long-lasting overexpression of the human CEP19 gene. This ciliary protein is critical for investigating the structural basis of morbid obesity; genome integration enables the establishment of stable cell lines for high-fidelity functional validation. Every batch is verified through comprehensive quality control screening (titer, sterility, and mycoplasma), ensuring that experimental results are both repeatable and safe.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: CEP19

Full Name: Centrosomal protein 19

Gene Symbol: MOSPGF; C3orf34

Gene ID: 84984

RefSeq ID-1: NP_001366398.1

RefSeq ID-2: NM_001379469.1

Summary: CEP19 encodes a protein that localizes to centrosomes and primary cilia, specifically associating with the mother centriole. While its molecular function is focused on cilia and centrosome integrity, this gene is located in a chromosomal region linked to severe obesity. Functional studies, particularly in mouse models where the orthologous gene is knocked out, demonstrate a profound metabolic phenotype, including morbid obesity, hyperphagy (excessive eating), glucose intolerance, and insulin resistance. In humans, mutations in CEP19 directly cause Morbid Obesity and Spermatogenic Failure (MOSPGF), underscoring the critical link between ciliary function and energy balance.

AdipoUpX™ Human CEP19 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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