AdipoUpX™ Human APOB Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human APOB Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human APOB gene. As the primary protein component of LDL and VLDL, its stable overexpression is an ideal tool for researching atherogenic lipid profiles and hepatic lipid export. The lentivirus characteristic of genome integration ensures a reliable cell model for studying lipid-related metabolic disorders. Comprehensive quality control is performed on all viral particle products to ensure both product safety and experimental efficacy.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: APOB

Full Name: Apolipoprotein B

Gene Symbol: FLDB; FCHL2; LDLCQ4; apoB-48; apoB-100

Gene ID: 338

RefSeq ID-1: NP_000375.3

RefSeq ID-2: NM_000384.3

Summary: APOB encodes the principal apolipoprotein of chylomicrons and low-density lipoproteins (LDL) and serves as the ligand for LDL receptors. It exists as two isoforms: apoB-48, produced in the gut, and apoB-100, produced in the liver. Both share a common N-terminal sequence, with apoB-48 generated via RNA editing of apoB-100 to introduce a stop codon. Mutations in APOB or its regulatory elements cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia, affecting plasma cholesterol and LDL levels.

AdipoUpX™ Human APOB Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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