LipoKnoxa™ Human KSR2 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX494-1	CCAACCAGCAGAGGCAATAAT	CDS	Inquiry
V0126XX494-2	CAGCCAGGGTGATGCGTTATT	3' UTR	Inquiry
V0126XX494-3	TGTGCGAGACTGTGGAGAAAT	CDS	Inquiry
V0126XX494-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human KSR2 shRNA Lentiviral Particle (Silencing) facilitates the stable knockdown of KSR2, a scaffold protein that regulates AMPK signaling and basal metabolic rate. Our lentiviral particles support dual expression strategies—U6 for high-potency silencing and miR30 for natural-mimicking processing—to meet diverse experimental needs. Rigorous quality control, including high-precision functional titering and mycoplasma detection, is performed on all particles to guarantee data safety.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: KSR2

Full Name: Kinase suppressor of ras 2

Gene ID: 283455

RefSeq ID-1: NP_775869.3

RefSeq ID-2: NM_173598.4

Summary: KSR2 encodes a serine/threonine kinase scaffold protein involved in Ras-mediated signaling, calcium-dependent pathways, and regulation of cold-induced thermogenesis. It functions upstream of the MAPK cascade and is localized in the cytosol and plasma membrane. Variants in KSR2 can disrupt energy balance and thermogenic responses, contributing to obesity.

LipoKnoxa™ Human KSR2 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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