AdipoUpX™ Human BBS9 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS9 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human BBS9 gene. As a key structural subunit of the BBSome, its stable overexpression is essential for researching the assembly of the ciliary machinery and its influence on satiety receptors. The lentivirus characteristic of genome integration establishes reliable cell models for long-term validation. Every batch undergoes rigorous internal quality control to ensure both product safety and the reproducibility of your experimental results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: BBS9

Full Name: Bardet-Biedl syndrome 9

Gene Symbol: B1; D1; C18; PTHB1

Gene ID: 27241

RefSeq ID-1: NP_001028776.1

RefSeq ID-2: NM_001033604.2

Summary: BBS9 encodes a protein that is a subunit of the BBSome complex, essential for ciliary function and trafficking. Its expression is known to be downregulated by parathyroid hormone in osteoblastic cells, suggesting a potential role in PTH signaling pathways within bone. While its precise function is still under investigation, mutations in BBS9 are implicated in Bardet-Biedl syndrome (BBS). As with all BBS genes, the resulting dysfunction in ciliary signaling leads to the characteristic BBS phenotype, which includes multi-organ defects and severe, monogenic obesity.

AdipoUpX™ Human BBS9 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products