AdipoUpX™ Human ALPL MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ALPL MSCV Particle (Overexpression) is a professionally designed vector particle for the stable overexpression of tissue-nonspecific alkaline phosphatase. This enzyme is increasingly recognized for its role in vascular calcification and metabolic health in the context of obesity. The MSCV structure is optimized for efficient integration in hematopoietic stem cells, stem cells, and diverse mammalian cell lines. Every batch undergoes comprehensive quality control, including titer and sterility testing, ensuring a high-performance tool for researchers investigating metabolic bone and adipose crosstalk.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ALPL

Full Name: Alkaline phosphatase, biomineralization associated

Gene Symbol: HOPS; HPPA; HPPC; HPPI; HPPO; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP; TNS-ALP

Gene ID: 249

RefSeq ID-1: NP_000469.3

RefSeq ID-2: NM_000478.5

Summary: The ALPL gene encodes tissue-nonspecific alkaline phosphatase, a membrane-bound enzyme essential for bone mineralization. It facilitates the hardening of the skeleton by regulating local phosphate levels. Clinical mutations in ALPL are the primary cause of hypophosphatasia, a disorder marked by hypercalcemia and significant skeletal defects.

AdipoUpX™ Human ALPL MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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