Sheep pro-opiomelanocortin (POMC) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1455

Assay Type: Quantitative competitive ELISA

Target Species: Sheep

Assay Target: POMC

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Sheep pro-opiomelanocortin (POMC) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Sheep pro-opiomelanocortin (POMC) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in sheep samples. The kit is highly sensitive and easy to use.

Assay Principle: This POMC ELISA Kit utilizes a competitive enzyme immunoassay technique involving a monoclonal anti-POMC antibody and a POMC-HRP conjugate. Samples and buffers are incubated with the conjugate in a pre-coated plate for one hour. During this period, POMC from the sample competes with the POMC-HRP conjugate for a limited number of antibody binding sites. After incubation, the wells are washed five times to remove unbound materials. An HRP-specific substrate is then added to form a blue complex that turns yellow upon the addition of a stop solution. The resulting color intensity, measured at 450 nm, is inversely proportional to the concentration of POMC in the sample. A standard curve is plotted to interpolate the final POMC concentration.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Sheep pro-opiomelanocortin (POMC) ELISA kit-Quantitative competitive is used to quantify POMC in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of sheep, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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