Mouse leptin (LEP) ELISA kit (5-2000 ng/L)

Cat. No.: 0126WXX-823

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: LEP

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Mouse leptin (LEP) ELISA kit (5-2000 ng/L)

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Product Overview

Description: Mouse leptin (LEP) ELISA kit (5-2000 ng/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in mice samples with a range of 5-2000 ng/L and a minimum detectable dose (sensitivity) of 2.57 ng/L. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit features a microplate pre-coated with a mouse LEP capture antibody. LEP in the sample binds to the immobilized antibody, followed by the binding of a biotinylated mouse LEP detection antibody and streptavidin-HRP. After incubation and a wash step to remove unbound reagents, a substrate solution is introduced to produce color in direct proportion to the concentration of mouse LEP. The reaction is stopped with an acidic solution and measured at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Mouse leptin (LEP) ELISA kit (5-2000 ng/L) is used to quantify LEP in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 5-2000 ng/L

Sensitivity: 2.57 ng/L

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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