Rat lipoprotein lipase (LPL) ELISA kit (0.625-40 ng/mL)

Cat. No.: 0126WXX-964

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LPL

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Rat lipoprotein lipase (LPL) ELISA kit (0.625-40 ng/mL)

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Product Overview

Description: Rat lipoprotein lipase (LPL) ELISA kit (0.625-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in rat samples with a range of 7.8-500 pg/mL and a minimum detectable dose (sensitivity) of <1.95 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. A microplate is pre-coated with an antibody specific to LPL. Standards and samples are pipetted into the wells, where any LPL present is immobilized by the capture antibody. After washing away unbound substances, a biotin-conjugated detection antibody specific for LPL is added. Following a subsequent wash, avidin-conjugated HRP is introduced. After a final wash to remove any unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of LPL bound in the initial step. The reaction is stopped, and the intensity of the color is measured to determine the sample concentration.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Rat lipoprotein lipase (LPL) ELISA kit (0.625-40 ng/mL) is used to quantify LPL in serum, plasma, cell culture supernatant, and tissue homogenates of rat, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Tissue homogenates

Detection Range: 7.8-500 pg/mL

Sensitivity: <1.95 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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