Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL)

Cat. No.: OB0525WXX-257

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ADP

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Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL)

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Product Overview

Description: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 0.156-10 ng/mL and a sensitivity of 0.061 ng/mL.

Assay Principle: The detection principle used in this kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit is pre-coated with an antibody specific for ADP. Standards or samples are then added to the corresponding microtiter plate wells along with the biotin-conjugated ADP-specific antibody. Avidin conjugated to HRP is then added to each microtiter plate well and incubated. Upon addition of the TMB substrate solution, only the wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.061 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.061 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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