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Vitamin B12 test kit

Rat fatty-acid amide hydrolase (FAAH) ELISA kit (0.156-10 ng/mL)

Cat. No.: OB0625WXX-806
Assay Type: Quantitative sandwich ELISA
Target Species: Rat
Assay Target: FAAH
Size:
Rat fatty-acid amide hydrolase (FAAH) ELISA kit (0.156-10 ng/mL)
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Product Overview

Description: Rat fatty-acid amide hydrolase (FAAH) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAAH in rat with a range of 0.156-10 ng/mL and a specificity of 0.039 ng/mL.
Assay Principle: An antibody specific for FAAH has been pre-coated onto the microtiter plate. Standards and samples are pipetted into the wells, and any FAAH present is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for FAAH is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of FAAH bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Background: FAAH degrades bioactive fatty acid amides into corresponding acids, thereby terminating the signaling function of these molecules. It participates in neural signaling pathways involved in regulating feeding and is associated with obesity.
Synonyms: FAAH-1; Anandamide amidohydrolase 1; Oleamide hydrolase 1; EC 3.5.1.99
EC NO.: 3.5.1.99
Applications: Rat fatty-acid amide hydrolase (FAAH) ELISA kit (0.156-10 ng/mL) is used to quantify FAAH in serum, plasma, tissue homogenate samples of rat, providing data to support a wide range of studies.
Research Area: Enzyme and Kinase; Fatty acid metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates
Detection Range: 0.156-10 ng/mL
Sensitivity: 0.039 ng/mL
Precision (Intra-assay): CV<8%
Precision (Inter-assay): CV<10%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.

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