Inquiry
Products
Online Inquiry

Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and treatment services for individuals.

Vitamin B12 test kit

Chicken glypican 4 (GPC4) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-256
Assay Type: Quantitative competitive ELISA
Target Species: Chicken
Assay Target: GPC4
Size:
Chicken glypican 4 (GPC4) ELISA kit-Quantitative competitive
INQUIRY Datasheet MSDS
Add to basket

Product Overview

Description: Chicken glypican 4 (GPC4) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GPC4 in chicken samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle: The GPC4 ELISA kit utilizes a competitive enzyme immunoassay technique featuring a monoclonal anti-GPC4 antibody and a GPC4-HRP conjugate. Samples and assay buffer are incubated with the conjugate in a pre-coated plate for one hour. Endogenous GPC4 and the HRP-conjugate compete for a limited number of antibody binding sites. After incubation, the wells are washed five times. An HRP substrate is then introduced, forming a blue complex that turns yellow upon adding a stop solution. The absorbance is measured at 450 nm. The color intensity is inversely proportional to the GPC4 concentration. A standard curve is plotted to relate the O.D. to standard concentrations for sample interpolation.
Background: GPC4 is a glycoprotein located on the cell membrane that participates in signaling pathways for multiple growth factors. It is implicated in the differentiation and proliferation of adipocytes. GPC4 may be associated with obesity by influencing the development of fat cells.
Synonyms: K-Glypican; Glypican proteoglycan 4; Secreted glypican-4
Applications: Chicken glypican 4 (GPC4) ELISA kit is used to quantify GPC4 in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of chicken, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.
Research Area: Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range: 0.5-10 ng/mL
Sensitivity: 0.1 ng/mL
Cross-reactivity: No significant cross-reactivity or interference was observed.
Recovery: 94-103%
Storage: Store at 2-8°C.

Related Products

Inquiry
0
Inquiry Basket

Recognizing the multifaceted nature of obesity, we leverage our expertise in preclinical research to provide tailored solutions that address the unique challenges faced by our partners in the biotechnology and pharmaceutical industries.

By Molecule Type

By Products

By Services

Contact Us

Copyright © Protheragen. All rights reserves.