Rat adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, <4.76 pg/mL)

Cat. No.: OB0525WXX-189

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: ACTH

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Rat adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, <4.76 pg/mL)

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Product Overview

Description: Rat adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, <4.76 pg/mL) is a ready-to-use ELISA kit for analyzing rat ACTH levels with a detection range of 15.6-1000 pg/mL. Its sensitivity is less than 4.76 pg/mL.

Assay Principle: The assay utilizes a competitive inhibitor enzyme immunoassay technique. A monoclonal antibody specific for ACTH is pre-coated on a microtiter plate. Biotin-labeled ACTH and unlabeled ACTH (standards or samples) undergo a competitive inhibition reaction with the pre-coated ACTH-specific antibody. After incubation, unbound conjugates are washed away. Next, avidin bound to HRP is added to each microtiter plate well and incubated. The amount of bound HRP is inversely proportional to the concentration of ACTH in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of ACTH in the sample.

Background: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.

Synonyms: ACTH; Corticotropin; Adrenocorticotropin; Adrenocorticotropic hormone; Pomc1; Pomc-1; Beta-LPH; AlphaMSH; beta-MSH; Gamma-LPH; Alpha-MSH; Gamma-MSH

Applications: Rat adrenocorticotropic hormone (ACTH) ELISA kit (15.6-1000 pg/mL, <4.76 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in rat serum, plasma, and other biological fluid samples.

Research Area: Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity

Specification

Sample Type: Rat serum; Plasma; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: <4.76 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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