Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.39-25 ng/mL)

Cat. No.: OB0525WXX-111

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: ACP5

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Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.39-25 ng/mL)

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Product Overview

Description: Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.39-25 ng/mL) is a ready-to-use ELISA kit for analyzing rat ACP5 levels with a detection range of 0.39-25 ng/mL and a sensitivity of 0.15 ng/mL. This kit has high sensitivity and specificity.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an ACP5-specific antibody. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin antibody specific for ACP5. HRP-conjugated avidin is then added to each well and incubated. Upon addition of TMB substrate solution, only wells containing ACP5, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ACP5 in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: ACP5 is involved in the dephosphorylation of proteins. Its expression appears to increase in certain pathological states. Mice overexpressing ACP5 have been found to exhibit hyperplastic obesity. There are two phenotypes of this enzyme: 5a and 5b. TRAP 5a binds to preadipocytes and may exert cell number-regulating activity, inducing preadipocyte proliferation/differentiation. TRAP 5b may also induce the proliferation of certain cell types.

Synonyms: HPAP; TRAP; TRACP5a; TRACP5b; TrATPase; Tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase; Tartrate-resistant acid phosphatase; TRAP 5; ACP 5; EC 3.1.3.2

Applications: Rat acid phosphatase 5, tartrate resistant (ACP5) ELISA kit (0.39-25 ng/mL) is designed for the in vitro quantitative analysis of ACP5 levels in rat serum, plasma, tissue homogenates, and other biological fluid samples.

Research Area: Hormone regulation; Metabolites; Defense response; Inflammatory; Obesity

Specification

Sample Type: Rat serum; Plasma; Tissue homogenates; Other biological fluids

Detection Range: 0.39-25 ng/mL

Sensitivity: 0.15 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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